NADH, or nicotinamide adenine dinucleotide in its reduced form, is a crucial coenzyme in various metabolic reactions, particularly in the processes of cellular respiration and energy production. The absorbance spectra of NADH are particularly important in biochemical assays, as they allow for the quantification of the molecule based on how much light it absorbs at specific wavelengths.
The absorbance maxima for NADH are specifically found at wavelengths of 340 nm and 366 nm. At 340 nm, NADH exhibits a strong absorbance peak which is often utilized in colorimetric assays to measure its concentration. The secondary peak at 366 nm is also significant for some applications but is less commonly referenced in standard absorbance measurements.
This understanding of the absorbance maxima is pivotal in enzymatic reactions and assays where NADH is involved, as it allows for the monitoring of the reaction fate and the estimation of enzyme kinetics using spectrophotometry.
The other options do not accurately reflect the correct absorbance maxima observed for NADH. For example, maxima at 300 and 400 nm are not characteristic of NADH, nor are those at 330 and 366 nm. Knowing the specific absorbance points for NADH is crucial for accurate laboratory analysis