Why is care necessary when preparing polyacrylamide gel?

Care is necessary when preparing polyacrylamide gel primarily because acrylamides are classified as carcinogenic. This means that exposure to acrylamide, often found in the gel preparation process, poses health risks to individuals handling the substance, making proper handling and safety protocols critical. Personal protective equipment (PPE), such as gloves and masks, is essential to reduce the risk of inhalation or skin contact. The preparation process often involves mixing acrylamide with a cross-linking agent, which can release harmful fumes, further emphasizing the importance of careful handling.

While the other aspects, such as moisture absorption, temperature sensitivity, and pH range, are relevant for successful gel preparation and performance, the primary concern when handling the chemicals involved is the carcinogenic nature of acrylamide, which necessitates meticulous care and adherence to safety protocols.