Which protein method is known for having the highest analytical sensitivity?

The Folin-Lowry method is recognized for its high analytical sensitivity in protein quantification. This method relies on the reduction of phosphomolybdic and phosphotungstic acids by phenolic compounds, which are released from proteins during hydrolysis. The resulting blue complex can be measured spectrophotometrically, allowing for the detection of very low concentrations of protein.

The sensitivity of the Folin-Lowry method primarily stems from its ability to detect proteins in the presence of various other substances, which often interferes with other assays. Its broad dynamic range enables the detection of nanogram quantities of protein, making it suitable for samples where the protein content is exceptionally low.

In comparison to other methods, such as the Bradford method, BCA assay, and Lowry method, the Folin-Lowry approach has a more sensitive response, particularly for proteins that contain aromatic amino acids that can participate in the reduction process. Therefore, when analyzing low protein concentrations, the Folin-Lowry method is preferentially used due to its high sensitivity and reliability in yielding reproducible results.