Which of the following is detected using high resolution electrophoresis?

High resolution electrophoresis is a sophisticated analytical technique that separates biomolecules based on size, charge, and shape, allowing for the detailed examination of various substances in biological samples. Among the options provided, the detection of light chains in urine, which is particularly relevant in the context of lymphoproliferative disorders, is best performed using high resolution electrophoresis.

In lymphoproliferative disorders, abnormal proliferation of lymphoid cells can lead to excessive production of certain proteins, including immunoglobulin light chains. High resolution electrophoresis can effectively differentiate the various light chains in urine, helping to identify monoclonal gammopathies and conditions such as multiple myeloma or other related disorders. This technique provides a clearer resolution of the proteins, allowing for better identification of abnormal patterns that are indicative of disease.

In contrast, other options do not utilize high resolution electrophoresis for their detection. Hemoglobin variants may also be detected through electrophoresis; however, the specific conditions and resolution might not necessitate high resolution. Cholesterol levels and blood glucose levels are typically measured using biochemical assays rather than electrophoretic methods, underscoring the specialized application of high resolution electrophoresis in protein analysis related to hematologic disorders.