What substrate is recommended by the IFCC for amylase?

The recommended substrate for measuring amylase activity according to the International Federation of Clinical Chemistry (IFCC) is p-nitrophenyl maltoheptoside. This substrate is specifically designed for the enzymatic reaction catalyzed by amylase, which hydrolyzes starch and other polysaccharides into simpler sugars. The use of p-nitrophenyl maltoheptoside allows for a colorimetric method to indirectly quantify amylase activity; when amylase acts on this substrate, it releases p-nitrophenol, which can be measured spectrophotometrically.

This substrate is favored because it provides a clear and sensitive measurement of amylase activity, facilitating more accurate diagnosis and monitoring of conditions associated with abnormal amylase levels, such as pancreatitis or salivary gland disorders. By utilizing a specific substrate, the assay can effectively differentiate amylase activity from other enzymes that may interfere with less specific substrates. The choice of a substrate with a clear assay endpoint enhances reliability and precision in laboratory measurements of amylase.