What is the order of hemoglobin migration on citrate agar at pH 6.2?

The order of hemoglobin migration on citrate agar at pH 6.2 is influenced by the differences in the electrical charge and structure of the various hemoglobin types during electrophoresis. Hemoglobin types will migrate at different rates based on their charge at the given pH, which affects their interaction with the gel.

In this particular scenario, the order of migration is determined by the relative ionic strength and the pH of the medium. At pH 6.2, hemoglobin variants with more negative charges will migrate faster towards the positive anode, while those with fewer negative charges will migrate slower.

The correct sequence identified in this context indicates that hemoglobin F (fetal hemoglobin) migrates ahead of hemoglobins A (adult) and S (sickle), followed by hemoglobin C. This particular order illustrates that hemoglobin F has a stronger negative charge compared to hemoglobin A, which is less negatively charged than hemoglobin S, and hemoglobin C is the least negatively charged, causing it to migrate the slowest.

Therefore, the order of migration of hemoglobin types on citrate agar at pH 6.2 is F-A-S-C. This sequence provides critical insight into the understanding of hemoglobin variants and their