What is the most common method for measuring uric acid in serum or plasma?

The most common method for measuring uric acid in serum or plasma is the peroxidase-coupled uricase reaction. This technique involves the enzyme uricase, which oxidizes uric acid to allantoin, producing hydrogen peroxide in the process. The hydrogen peroxide can then react with a chromogenic substance in the presence of peroxidase to produce a color change that can be quantitatively measured. This method is advantageous because it offers specificity for uric acid, allowing for accurate measurements without interference from other substances present in the serum or plasma.

The peroxidase-coupled uricase reaction is widely used in clinical laboratories due to its reliability and consistency in producing results. While other methods like colorimetric assays and HPLC can also measure uric acid, the peroxidase-coupled uricase reaction is preferred in many settings for its efficacy and straightforward interpretation of results. This technique encapsulates a well-established biochemical pathway that enhances accuracy in uric acid quantification, critical for diagnosing conditions linked with uric acid metabolism, such as gout and kidney disease.