What is the method of choice for quantitatively determining Hgb A2 and Hgb F?

High-performance liquid chromatography (HPLC) is recognized as the method of choice for quantitatively determining hemoglobin A2 (Hgb A2) and hemoglobin F (Hgb F) due to its high sensitivity, specificity, and ability to separate components in a complex mixture.

In the context of hemoglobin analysis, HPLC allows for precise quantification of different hemoglobin types by passing a sample through a column under high pressure. The separation of Hgb A2 and Hgb F from other hemoglobin types occurs based on their differing characteristics, such as size and charge, which enables accurate measurement.

Additionally, HPLC is capable of providing a clear, quantitative assessment of hemoglobin variants in a short timeframe, which is essential in clinical settings for diagnosing conditions such as thalassemias or sickle cell disease. While methods like capillary electrophoresis, gel electrophoresis, and mass spectrometry have their own advantages, they may not provide the same level of specificity and quantitation for Hgb A2 and Hgb F as HPLC does, especially in routine clinical applications.